Methods and compositions for detection of microbial contaminants in peritoneal dialysis solutions

摘要

Methods and compositions for detection of microbial contaminants in peritoneal dialysis solutions are provided. A novel cause of aseptic peritonitis is provided—aseptic peritonitis associated with gram positive microbial contamination of a dialysis solution. Peptidoglycan is a major component of a gram positive bacterial cell wall and thus can serve as a marker for gram positive bacteria. In this regard, testing for peptidoglycans can be utilized to effectively prevent peritonitis in patients that use the peritoneal dialysis solutions, such as peritoneal dialysis solutions that contain a glucose polymer including an icodextrin and the like.

主权项

1. A method for manufacturing a dialysis solution, the method comprising:
providing a glucose polymer; performing a modified bioburden test on the glucose polymer to detect contaminating Alcyclobacillus acidocaldarius; testing the glucose polymer with a reagent that is reactive with any peptidoglycan in the glucose polymer to determine a peptidoglycan concentration; and using the glucose polymer to prepare the dialysis solution if the peptidoglycan concentration is determined to be about 10 ng/mL or less.