发明名称 |
Nucleic acid amplification primers for PCR-based clonality studies |
摘要 |
The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-JK IGK rearrangement, a VK/intron-Kde IGK rearrangement, a Vλ-Jλ IGL rearrangement, a Vβ-Jβ TCRB rearrangement, a Dβ-Jβ TCRB rearrangement, a Vγ-Jγ TCRG rearrangement, a Vδ-Jδ TCRD rearrangement, a Dδ-Dδ TCRD rearrangement, a Dδ-Jδ TCRD rearrangement, a Vδ-Dδ TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention. |
申请公布号 |
US8859748(B2) |
申请公布日期 |
2014.10.14 |
申请号 |
US200310531106 |
申请日期 |
2003.10.13 |
申请人 |
|
发明人 |
Van Dongen Jacobus Johannes Maria;Langerak Anthonie Willem;Schuuring Eduardus Maria Dominicus;San Miquel Jesus Fernando;Garcia Sanz Ramon;Parreira Antonio;Smith John Lewis;Lavender Frances Louise;Morgan Gareth John;Evans Paul Anthony Stuart;Kneba Michael;Hummel Michael;Macintyre Elizabeth Anne;Bastard Christian |
分类号 |
C07H21/04;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
Knobbe, Martens, Olson & Bear, LLP |
代理人 |
Knobbe, Martens, Olson & Bear, LLP |
主权项 |
1. A kit for the detection of at least one IGH rearrangement, wherein said kit comprises a plurality of forward primers selected from the group consisting of SEQ ID NOs: 1-8, 10-15, 17-20, and 22-28, and at least one reverse primer comprising SEQ ID NO:21, wherein at least one of said plurality of forward primers or at least one of said at least one reverse primer comprises a fluorescent label. |
地址 |
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