Polypeptides having beta-glucosidase activity and polynucleotides encoding same

摘要

The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

主权项

1. A nucleic acid construct or recombinant expression vector comprising an isolated polynucleotide encoding a polypeptide, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, wherein the polypeptide having beta-glucosidase activity is selected from the group consisting of:
(a) a polypeptide having at least 95% sequence identity to amino acids 19 to 776 of SEQ ID NO: 4; (b) a polypeptide encoded by a polynucleotide that hybridizes under at least very high stringency conditions with (i) nucleotides 55 to 2557 of SEQ ID NO: 3 or a cDNA sequence thereof, or (ii) the full-length complement of (i), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.; (c) a polypeptide encoded by a polynucleotide having at least 99% sequence identity to nucleotides 55 to 2557 of SEQ ID NO: 3 or a cDNA sequence thereof; and (d) an isolated fragment of the polypeptide of (a), (b), or (c) that has beta-glucosidase activity.