发明名称 |
METHODS FOR TRANSFORMING AND EXPRESSION SCREENING OF FILAMENTOUS FUNGAL CELLS WITH A DNA LIBRARY |
摘要 |
The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides. |
申请公布号 |
CA2565888(C) |
申请公布日期 |
2014.10.14 |
申请号 |
CA20052565888 |
申请日期 |
2005.05.26 |
申请人 |
NOVOZYMES, INC. |
发明人 |
TETER, SARAH;LAMSA, MICHAEL;CHEERY, JOEL;WARD, CONNIE |
分类号 |
C12P21/06;C12N1/16;C12N15/74 |
主分类号 |
C12P21/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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