发明名称 |
Fully human hybridoma fusion partner cell lines |
摘要 |
Certain aspects of the present invention are directed to new fully human fusion partner cell lines called human Karyochi cells, and to methods for making them. Human Karyochi cells are then fused with human antibody-secreting lymphoid cells to make fully human hybridomas called Karyochi-based hybridomas, which likewise secrete fully human monoclonal antibodies. Human Karyochi cells are made by isolating a donor nucleus that is substantially free of cytoplasm from either a first malignant B-lymphocyte cell line or a normal B-lymphocyte, and transferring the donor nucleus into the cytoplasm of a recipient cell from a second T- or B-lymphoid cell line. With time the nuclei synchronize and fuse to form the chimeric Karyochi fusion partner cell line. Nuclear transfer can be accomplished using intra-cytosolic nucleus injection or by impact-induced nucleus administration. |
申请公布号 |
US8859278(B2) |
申请公布日期 |
2014.10.14 |
申请号 |
US200611510490 |
申请日期 |
2006.08.25 |
申请人 |
The Trustees of Columbia University in the City of New York |
发明人 |
Trakht Ilya;Kalantarov Gavreel |
分类号 |
C12N5/0781;C12N5/09;C12N5/10;C07K16/00;C12N5/16 |
主分类号 |
C12N5/0781 |
代理机构 |
Evans & Molinelli PLLC |
代理人 |
Evans & Molinelli PLLC ;Evans Judith |
主权项 |
1. The human lymphoma cell line FP0 having ATCC Patent Deposit Designation Number PTA-7466. |
地址 |
New York NY US |