Method for modulating gene expression by modifying the CpG content

摘要

The invention relates to nucleic acid modifications for a directed expression modulation by the targeted insertion or removal of CpG dinucleotides. The invention also relates to modified nucleic acids and expression vectors.

主权项

1. An isolated nucleic acid molecule having a region capable of transcription, codon-optimized relative to an expression system, wherein the number of CpG dinucleotides is increased compared to the codon-optimized sequence without altering the amino acid sequence encoded by said isolated nucleic acid molecule.