Fusion constructs and use of same to produce antibodies with increased Fc receptor binding affinity and effector function

摘要

The present invention relates to the field of glycosylation engineering of proteins. More particularly, the present invention relates to nucleic acid molecules, including fusion constructs, having catalytic activity and the use of same in glycosylation engineering of host cells to generate polypeptides with improved therapeutic properties, including antibodies with increased Fc receptor binding and increased effector function.

主权项

1. A method for modifying the glycosylation of a polypeptide produced by a host cell, comprising introducing into said host cell a nucleic acid comprising a sequence encoding a fusion polypeptide, wherein said fusion polypeptide has β(1,4)-galactosyltransferase activity and comprises a Golgi localization domain selected from the group consisting of: the localization domain of mannosidase II, the localization domain of β(1,2)-N-acetylglucosaminyltransferase I, the localization domain of mannosidase I, the localization domain of β(1,2)-N-acetylglucosaminyltransferase II, and the localization domain of α1-6 core fucosyltransferase, and wherein said modified polypeptide has increased Fc-receptor binding or effector function as a result of said modification.