Genome wide identification and characterization of gene expression regulatory elements in <i>Zea mays </i>for use in plants

摘要

The present invention provides non-coding regulatory element polynucleotide molecules isolated from the lipid transfer protein (LTP) gene of Oryza sativa and useful for expressing transgenes in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the Oryza sativa regulatory polynucleotide sequences, and methods for preparing and using the same.

主权项

1. A polynucleotide molecule comprising:
i) a regulatory polynucleotide molecule comprising a nucleic acid sequence selected from the group consisting of:
(a) a nucleic acid sequence that exhibits 98% or greater identity to SEQ ID NO:4756 and has promoter activity;(b) a fragment comprising at least 95 contiguous nucleotides of SEQ ID NO: 4756 and has promoter activity; and(c) the nucleic acid sequence of SEQ ID NO:4756; and ii) a heterologous transcribable polynucleotide molecule, wherein the regulatory polynucleotide molecule is operably linked to the heterologous transcribable polynucleotide molecule.