Micro-device and methods for disrupting cells

摘要

A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber

主权项

1. A method of disrupting cells, the method comprising:
introducing the cells into a first chamber of a micro-device for disrupting cells, wherein the micro-device comprises:
the first chamber;a second chamber, which is pressurized and depressurized by flowing a fluid into and out of the second chamber through a port;a flexible membrane which-separates the first chamber and the second chamber and is vibrated by pressurizing and depressurizing the second chamber; anda micro-unit for disrupting cells confined in the first chamber; disrupting the cells by vibrating the flexible membrane between the first chamber and the second chamber, after the introducing the cells; and releasing resultant disrupted cells from the first chamber of the micro-device; wherein the flexible membrane is vibrated by periodically or non-periodically adjusting the pressure of the interior of the second chamber such that the flexible membrane is vibrated, wherein the micro-device comprises: an upper plate with a space defining the first chamber; and a lower plate with a space defining the second chamber; wherein the flexible membrane is disposed between the upper plate and the lower plate, the first chamber is delimited by the upper plate and the flexible membrane, and the second chamber is delimited by the lower plate and the flexible membrane.