Genetically encoded photomanipulation of protein and peptide activity

摘要

The present invention relates to fusion proteins comprising protein light switches and methods of photomanipulating the activity of the proteins. The invention further relates to polynucleotides and vectors encoding the fusion proteins, cells comprising the fusion proteins, and methods of using the fusion proteins to study protein function and analyze subcellular activity, as well as diagnostic and therapeutic methods.

主权项

1. A fusion protein comprising a protein of interest fused to a protein light switch comprising a light, oxygen or voltage (LOV) domain, wherein illumination of the fusion protein activates or inactivates the protein of interest, and wherein the protein of interest and the protein light switch are not found fused together in nature.