Célula de levadura con un vector de expresión que comprende un polinucleótido con la secuencia de la proteina de cápside de vp2 del virus de la necrosis pancreática infecciosa (ipnv) y con la secuencia de uno o mas epítopos de la hemaglutinina del virus de la anemia infecciosa del salmón; alimento para peces que comprende la célula de levadura; vacuna oral para peces.

摘要

The present invention provides bivalent oral vaccines against infectious pancreatic necrosis virus (IPNV) and infectious salmon anemia virus (ISAV). Yeast cells comprise an expression vector comprising (i) a polynucleotide sequence encoding a VP2 capsid protein of IPNV and (ii) a polynucleotide sequence encoding one or more antigenic epitopes of hemaglutinin of ISAV. The yeast cells express sub-viral particles (SVP) comprising a VP2 capsid protein of IPNV and one or more antigenic epitopes of hemaglutinin of ISAV. The yeast cells and SVP may be administered in an effective amount to increase the amount of antibodies against IPNV and ISAV in the fish, preferably Salmonidae. The yeast cells and SVP may be in the form of fish food for administering to the fish orally.