| 摘要 |
FIELD: medicine, pharmaceutics.SUBSTANCE: invention relates to the pharmaceutical industry, namely to a biologically active complex for organogenesis. The biologically active complex for organogenesis, is characterised by the fact, that a three-dimensional multi-component structure is organised by a three dimensional layer-by-layer replication of a native tissue of a human or animal structure, for the regeneration of which it is applied, for this purpose biopsy of the recipient's tissue is carried out, and in case of the absence of the tissue section with a need of its substitution an amino-acid composition of a protein component is determined by confocal microscopy, scanning and transmission electronic microscopy, a supramolecular structure is identified by a protein analyser, the composition of glycoseaminoglycanes and lipids is determined by the method of atomic-absorption analysis, then all basic protein components of the complex are synthesised by an autosynthesiser of proteins, glycoseaminoglycanes and lipids are selected qualitatively and quantitatively in ratios, maximally close to the ones obtained in biopsy analysis, then all the components are applied on a polymer substrate layer-by-layer, with the formation due to aggregation of the structure, similar to the recipient's own tissue, stem allogenic or autologous cells, covered by the following layer of proteins, lipids and glycoseaminoglycanes, are placed on each layer, the obtained complex is incubated and transplanted, closing the defect of the recipient's tissue.EFFECT: complex described above makes it possible to reduce the period of rehabilitation due to the absence of a tissue, cellular and immune response of the recipient and, accordingly, fibrous changes in the area of the complex implantation.10 ex |
