CELL SEPARATION METHOD

摘要

The present invention provides methods and compositions for separating cells from a sample containing erythrocytes. The method is for recovering desired cells from a sample containing the desired cells, erythrocytes and undesired cells comprising: a) contacting the sample with a composition, said composition comprising: i) an erythrocytes aggregation reagent ii) at least one antigen recognizing moiety coupled to a magnetic particle, wherein said particle with said at least one antigen recognizing moiety specifically binds to at least one antigen specific for one or more undesired cellular components; b) applying simultaneously i) gravity sedimentation for sedimentation of erythrocytes and ii) a magnetic field gradient to said sample for immobilizing said magnetic particle generating a pellet and a supernatant phase, and c) recovering the desired cells from the supernatant phase. Compositions for the use within the present method are also disclosed.

主权项

1. A method for enriching and/or recovering desired cells from a sample containing the desired cells, erythrocytes, and other undesired cell types such as leukocyte subpopulations and/or platelets, the method comprising:
a. contacting the sample with a composition, said composition comprising:
i. a reagent that causes erythrocytes to aggregate; andii. a set of one or more antigen recognizing moieties coupled to magnetic particles such that the magnetic particles specifically bind to one or more antigens that occur selectively on one or more undesired cell types; b. applying simultaneously
i. gravity sedimentation for sedimentation of erythrocytes; andii. a magnetic field gradient to said sample for immobilizing said magnetic particle thereby generating a pellet and a supernatant phase, and c. recovering the desired cells from the supernatant phase.