Compounds for Modulating Integrin CD11B/CD18

摘要

The application describes an assay for the identification of small molecule modulators of integrin CD11b/CD18 and small molecules capable of modulating activity of this receptor. Such compounds may be used in certain embodiments for treating a disease or condition selected from inflammation, immune-related disorders, cancer, ischemia-reperfusion injury, stroke, neointimal thickening associated with vascular injury, bullous pemphigoid, neonatal obstructive nephropathy, and cardiovascular disease, or in other embodiments for the treatment of a disease or condition selected from immune deficiency, acquired immune deficiency syndrome (AIDS), myeloperoxidase deficiency, Wiskott-Aldrich syndrome, chronic granulomatous disease, hyper-IgM syndromes, leukocyte adhesion deficiency, Chediak-Higashi syndrome, and severe combined immunodeficiency.

主权项

1. An assay for the identification of small molecule modulators of integrin CD11b/CD18, the assay comprising:
contacting cells with a solution comprising a test compound on a substrate treated with a compound that affects cell adhesion; physically repositioning the substrate such that non-adherent cells move away from the substrate by the action of gravity; and detecting adherent cells on the substrate.