MUTANT REPLICON DERIVED FROM GENOME OF HEPATITIS C VIRUS J6CF STRAIN

摘要

A nucleic acid includes the 5′ untranslated region, a virus protein-coding region including the NS3 protein coding sequence, the NS4A protein coding sequence, the NS4B protein coding sequence, the NS5A protein coding sequence, and the NS5B protein coding sequence, and the 3′ untranslated region of the HCV J6CF genome in that order from the 5′ to 3′ direction. The NS4A protein coding sequence has a mutation for substituting alanine at position 1680 with glutamic acid, as determined on the basis of the amino acid sequence of the precursor protein of the J6CF strain.

主权项

1. A nucleic acid comprising a 5′ untranslated region, a virus protein-coding region which contains an NS3 protein coding sequence, an NS4A protein coding sequence, an NS4B protein coding sequence, an NS5A protein coding sequence, and an NS5B protein coding sequence, and a 3′ untranslated region of the genome of the hepatitis C virus J6CF strain in that order from the 5′ to 3′ direction, wherein the NS4A protein coding sequence comprises a mutation substituting alanine at position 1680 with glutamic acid, as determined on the basis of the amino acid sequence as shown in SEQ ID NO: 30 of the precursor protein of the J6CF strain.