Method of obtaining a population of cells

摘要

A method of obtaining stromal progenitor cells (SPC) from subcutaneous adipose tissue by incubation of a very small volume of the subcutaneous adipose tissue in an enzyme solution produces SPC that are usable in medical applications based on autologous SPC even on individuals having a body mass index lower than 18.5.

主权项

1. A method of obtaining a population of stromal progenitor cells comprising the steps of:
collecting a first quantity q1 of subcutaneous adipose tissue containing white adipose cells from a living being; digesting the first quantity q1 with a second quantity q2 of enzymatic buffer to obtain a population of free cells; seeding the population of free cells into culture media needed for their expansion; and extracting stromal progenitors cells from the adipose tissue.