发明名称 |
PROCESS FOR PRODUCING CELLULASES USING A FILAMENTOUS FUNGUS SUITABLE FOR A FERMENTER, HAVING A LOW VOLUMETRIC OXYGEN TRANSFER COEFFICIENT KLA |
摘要 |
The present invention concerns a process for the production of cellulases using a strain of a filamentous fungus in a stirred, aerated bioreactor, comprising at least two growth steps:
the first growth step in the presence of at least one carbonaceous growth substrate in batch phase with a concentration of carbonaceous growth substrate in the range 10 to 80 g/L;a second step for growth and production in the presence of at least one inducer carbonaceous substrate in fed batch phase in the presence of a limiting flow of carbon source in the range 50 to 140 mg per gram of cells per hour. |
申请公布号 |
US2014302587(A1) |
申请公布日期 |
2014.10.09 |
申请号 |
US201214239690 |
申请日期 |
2012.08.02 |
申请人 |
Ben Chaabane Fadhel;Jourdier Etienne;Cohen Celine;Chaussepied Bernard |
发明人 |
Ben Chaabane Fadhel;Jourdier Etienne;Cohen Celine;Chaussepied Bernard |
分类号 |
C12N9/42 |
主分类号 |
C12N9/42 |
代理机构 |
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代理人 |
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主权项 |
1. A process for the production of cellulases using a strain belonging to a filamentous fungus in a stirred, aerated bioreactor, comprising at least two steps:
the first growth step in the presence of at least one carbonaceous growth substrate in batch phase, carried out with a concentration of carbonaceous growth substrate in the range 10 to 60 g/L; a second step for growth and enzyme production in the presence of at least one inducer carbonaceous substrate in fed batch phase in the presence of a limiting flow of carbon source in the range 50 to 140 mg per gram of cellular biomass per hour. |
地址 |
Paris FR |