GPCR Fusion Protein Containing an N-Terminal Autonomously Folding Stable Domain, and Crystals of the Same

摘要

Certain embodiments provide a GPCR fusion protein. In particular embodiments, the GPCR fusion protein comprises: a) a G-protein coupled receptor (GPCR); and b) an autonomously folding stable domain, where the autonomously folding stable domain is N-terminal to the GPCR and is heterologous to the GPCR. The GPCR fusion protein is characterized in that is crystallizable under lipidic cubic phase crystallization conditions. In certain embodiments, the GPCR fusion protein may be crystallizable in a complex with a G-protein or in a complex with an antibody that binds to the IC3 loop of the GPCR.

主权项

1. A GPCR fusion protein comprising,
a) a G-protein coupled receptor (GPCR); and, b) an autonomously folding stable domain; wherein said autonomously folding stable domain is N-terminal to said GPCR and is heterologous to said GPCR; and wherein said GPCR fusion protein is characterized in that is crystallizable under lipidic cubic phase crystallization conditions.