发明名称 |
METHODS FOR ENHANCED PROTEIN PRODUCTION |
摘要 |
The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×106 cells/mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer. |
申请公布号 |
US2014302581(A1) |
申请公布日期 |
2014.10.09 |
申请号 |
US201414309308 |
申请日期 |
2014.06.19 |
申请人 |
MEDAREX, L.L.C. |
发明人 |
Arunakumari Alahari;Dai Xiao-Ping;Garcia Javier;Martel Richard P. |
分类号 |
C07K1/30;C07K16/00 |
主分类号 |
C07K1/30 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method for clarifying a protein from a cell culture, comprising:
(A) adjusting the pH of the cell culture to below neutral pH; and (B) settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer. |
地址 |
Princeton NJ US |