SYSTEMS AND METHODS FOR DETERMINING GENETIC DATA

摘要

Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.

主权项

1. A method for sequencing a nucleic acid sample, comprising:
(a) associating a plurality of oligonucleotide analogs with said nucleic acid sample; and (b) directing the nucleic acid sample through a nanopore, wherein an individual oligonucleotide analog of the plurality of oligonucleotide analogs becomes disassociated from the nucleic acid sample as the sequence of the nucleic acid sample is determined,
wherein the plurality of oligonucleotide analogs that are associated with the nucleic acid sample comprise at least about 25% universal bases.