METHOD AND QUALITY CONTROL MOLECULAR BASED MOUSE EMBRYO ASSAY FOR USE WITH IN VITRO FERTILIZATION TECHNOLOGY

摘要

A method for qualitatively assessing products used in in vitro fertilization is provided. Also disclosed is an improved quality control assay for use in clinical Assisted Reproductive Technologies (ART).

主权项

1. A method for assessing a product used for Assisted Reproductive Technologies (ART), the method comprising:
providing a transgenic embryo, wherein said embryo comprises at least one reporter gene operably linked to the regulatory region of at least one embryonic viability marker and/or at least one transgene operably linked to the regulatory region of at least one embryonic viability marker; culturing said embryo in vitro for a specified duration; evaluating expression of the at least one reporter gene and/or the transgene during at least one stage of development of said embryo; and determining the acceptability or failure of said product based upon said evaluation, wherein the product is used in the method in a manner similar to its intended use in ART.