| 摘要 |
A liquid chromatography/mass spectrometry system includes: a source of a first mobile phase solvent consisting essentially of water plus 10 mM ammonium formate plus 0.05% formic acid; a source of a second mobile phase solvent consisting essentially of methanol plus 10 mM ammonium formate plus 0.05% formic acid; a chromatography column comprising a length of 30 mm or less of a stationary phase comprising an 8-carbon alkyl chain material bonded to 2.6 μm diameter particles having solid silica cores surrounded by porous silica outer layers; an electrospray ion source of a mass spectrometer fluidically coupled to the chromatography column so as to generate ions therefrom; a mass analyzer of the mass spectrometer operable to quantitatively detect the ions; and a programmable processor electronically coupled to the mass analyzer and comprising instructions operable to determine, based on the ion detection, a concentration of everolimus, sirolimus, tacrolimus, or cyclosporin A. |
| 主权项 |
1. A liquid chromatography/mass spectrometry system, comprising:
a source of a first mobile phase solvent (MPA) consisting essentially of water plus 10 mM ammonium formate plus 0.05% formic acid; a source of a second mobile phase solvent (MPB) consisting essentially of methanol plus 10 mM ammonium formate plus 0.05% formic acid; a length of tubing operable to receive a liquid sample therein; a chromatography column comprising a length of 30 mm or less of a stationary phase comprising an 8-carbon alkyl chain material bonded to 2.6 μm diameter particles having solid silica cores surrounded by porous silica outer layers; a first and a second fluidic pump operable to respectively propel a first flow of the MPA solvent and a first flow of the MPB solvent through, in sequence, a first fluidic junction, the length of tubing and the chromatography column; a third and a fourth fluidic pump operable to respectively propel a second flow of the MPA solvent and a second flow of the MPB solvent through, in sequence, a second fluidic junction and the chromatography column; a valve operable to transfer either the flows propelled by the first and second fluidic pumps or the third and fourth fluidic pumps or a mixture thereof to the chromatography column; an electrospray ion source of a mass spectrometer fluidically coupled to the chromatography column so as to receive compounds eluting from the chromatography column and to generate ions therefrom; a mass analyzer of the mass spectrometer operable to separate the generated ions according to their mass-to-charge (m/z) ratios and to quantitatively detect ions of each m/z ratio within a range of such ratios; and a programmable processor electronically coupled to the mass analyzer and comprising instructions operable to determine, based on the ion detection, a concentration of everolimus, sirolimus, tacrolimus, or cyclosporin A in the sample. |
