发明名称 |
METHODS FOR THE PHENOTYPIC DETECTION OF HCV INHIBITOR RESISTANT SUBPOPULATIONS |
摘要 |
<p>Methods and compositions for the efficient and accurate determination of susceptibility of a hepatitis C virus (HCV) population to an HCV inhibitor are provided. In certain aspects, the methods involve introducing into a cell a patient derived segment, wherein the cell or the patient derived segment comprises an indicator nucleic acid that produces a detectable signal that is dependent on the HCV; measuring the expression of the indicator gene in the presence of varying concentrations of the HCV inhibitor; determining a standard curve of susceptibility; comparing the IC95 fold change, slope, or maximum inhibition percentage of the HCV population to that of a control HCV population, and determining that the HCV population comprises HCV with a reduced susceptibility to the inhibitor when the IC95 fold change value is increased or the slope and/or maximum inhibition percentage is lower for the HCV population as compared to the control population.</p> |
申请公布号 |
EP2785876(A1) |
申请公布日期 |
2014.10.08 |
申请号 |
EP20120852521 |
申请日期 |
2012.12.03 |
申请人 |
LABORATORY CORPORATION OF AMERICA HOLDINGS |
发明人 |
REEVES, JACQUELINE DENISE;PETROPOULOS, CHRISTOS JOHN;HUANG, WEI |
分类号 |
C12Q1/70;G01N33/576 |
主分类号 |
C12Q1/70 |
代理机构 |
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代理人 |
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地址 |
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