NOVEL BACILLUS THURINGIENSIS GENE ENCODING CRYSTAL PROTEIN HAVING IMPROVED INSECTICIDAL ACTIVITY AGAINST LEPIDOPTERAN INSECTS AND PREPARING METHOD THEREOF

摘要

The present invention relates to a novel bacillus thuringiensis endotoxic protein gene with improved insecticidal activities against lepidopteran insects and to a method for producing the same. More specifically, the present invention relates to a method for producing a bacillus thuringiensis endotoxic gene with strong insecticidal activities against lepidopteran insects such as Mamestra brassicae, Spodoptera litura, and Spodoptera exigua. The purpose of the present invention is achieved through the steps of: confirming a base sequence and a tertiary structure of a mod-cry1AC gene; producing a PIM-mod-cry1AC vector using the mod-cry1AC gene obtained from the previous step; producing a mutation-inducing primer using the mod-cry1AC as a mold; performing a polymerase chain reaction (PCR) using the mutation-inducing primer obtained from the previous step so as to produce novel BT endotoxic protein gene to be transformed in colon bacilli and confirming mutations; and confirming a base sequence and a tertiary structure of the novel BT endotoxic protein gene obtained from the previous step. Accordingly, in the present invention, agricultural products, which are not damaged by larvae of various lepidopteran insects, can be produced using a microorganism formulation including novel recombinant endotoxic protein gene Mut-N16.