Treatment of inflammatory bowel disease

摘要

A method of treating inflammatory bowel disease (IBD) comprises collecting regulatory T cells in an activated or non-activated state from a patient's sentinel lymph nodes draining bowel segments with or without IBD, optionally activating the cells by contacting them with a cytokine and an antigen extract obtained from an inflamed bowel segment, expanding the T cells in vitro, and re-infusing the expanded T cell to the patient. Also disclosed are methods for obtaining sentinel nodes, for expanding T cells, for re-establishing the TH1/TH2 balance in the patient suffering from Crohn's disease, and corresponding uses of the expanded T cells, the cytokine and of antigen extract, as well as of correspondingly activated and expanded T cells.

主权项

1. A method of treating inflammatory bowel disease (IBD) in a human patient, comprising:
injecting a lymphatic staining agent into lesional areas of an intestine of a human patient suffering from IBD, identifying lymphatic tissue stained by the agent as sentinel lymph node draining bowel, isolating regulatory T cells from the sentinel lymph nodes, expanding the collected T cells in vitro in culture medium, contacting the collected T cells with one or more cytokines in combination with an antigen extract obtained from an inflamed bowel segment before, concurrently or after expansion, and re-infusing the expanded T cells to the patient.