Interpenetrating polymer network comprising fibrin

摘要

There is provided a method of forming a hydrogel, the method comprising: providing a mixture of a polymer comprising a cross-linkable pendant phenolic group, peroxidase, H2O2, fibrinogen, and thrombin, at concentration sufficient to enzymatically cross-link the polymer and to cleave the fibrinogen to yield fibrin; and allowing the mixture to form a hydrogel. There is also provided a hydrogel comprising a cross-linked network of a polymer interpenetrated by fibrin fibers, the polymer cross-linked by oxidative coupling between phenolic groups pendant on the polymer.

主权项

1. An angiogenesis support hydrogel comprising a cross-linked network of a hyaluronic-tyramine conjugate interpenetrated by fibrin fibers, the hyaluronic-tyramine conjugate cross-linked by oxidative coupling between tyramine groups, the hydrogel formed from a mixture comprising the hyaluronic-tyramine conjugate at a concentration of 1 mg/ml to 10 mg/ml, a peroxidase at a concentration of 0.05 unit/ml to 0.5 unit/ml, hydrogen peroxide at a concentration of 20 μM to 500 μM, fibrinogen at a concentration of 1 mg/ml to 10 mg/ml, and thrombin at a concentration of 0.1 unit/ml to 1.0 unit/ml.