Methods of inducing tissue regeneration

摘要

Methods are provided for producing cells within a lineage (lineage restricted cells) from post-mitotic differentiated cells of the same lineage ex vivo and in vivo, and for treating a subject in need of tissue regeneration therapy by employing these lineage-restricted cells. In addition, the production of lineage restricted cells from postmitotic tissues derived from patients with diseases allows for a characterization of pathways that have gone awry in these diseases and for screening of drugs that will ameliorate or correct the defects as a means of novel drug discovery. Also provided are kits for performing these methods.

主权项

1. A method of producing lineage-restricted mammalian cells (LRCs) from a post-mitotic differentiated mammalian cell (PMD) of the same lineage, comprising:
contacting a primary culture mammalian myocyte or hepatocyte PMD with an effective amount of a combination of agents comprising a first agent that transiently inhibits the activity of a pocket protein and an effective amount of a second agent that transiently inhibits the activity of the cyclin-dependent kinase inhibitor 2A alternate reading frame protein (ARF), under conditions sufficient to permit the PMD to transiently divide to produce progeny, wherein said progeny are LRCs of the same lineage as the PMD.