Consensus sequences of chikungunya viral proteins, nucleic acid molecules encoding the same, and compositions and methods for using the same

摘要

Consensus CHIKV E1 protein, consensus CHIKV E2 protein, consensus CHIKV capsid protein, or fragments and homologues thereof, and nucleic acid molecules that encode the same are disclosed. A consensus CHIKV Env protein which includes CHIKV E1 consensus protein, CHIKV E2 consensus protein, CHIKV E3 consensus protein, or fragments and homologues thereof and nucleic acid molecules that encode the same are also disclosed. Compositions and recombinant vaccines comprising CHIKV consensus proteins, and methods of using them are disclosed.

主权项

1. The DNA plasmid comprising a nucleic acid sequence selected from the group consisting of:
a nucleic acid sequence that encodes a consensus CHIKV Env including CHIKV E1 consensus protein or an immunogenic consensus fragment of CHIKV E1 consensus protein having at least 395 amino acids of CHIKV E1 consensus protein, CHIKV E2 consensus protein or an immunogenic consensus fragment of CHIKV E2 consensus protein having at least 380 amino acids of CHIKV E2 consensus protein, and CHIKV E3 consensus protein, wherein the nucleic acid-sequence that encodes CHIKV Env consensus protein comprises a nucleic acid sequence that encodes SEQ ID NO: 14 or SEQ ID NO:16; a nucleic acid sequence that encodes a consensus CHIKV E1 protein, wherein the nucleic acid-sequence that encodes CHIKV E1 consensus protein comprises SEQ ID NO: 1 or SEQ ID NO:4; a nucleic acid sequence that encodes an immunogenic consensus fragment of consensus CHIKV E1 protein having at least 395 amino acids of CHIKV E1 consensus protein, wherein the nucleic acid-sequence that encodes CHIKV E1 consensus protein comprises SEQ ID NO: 1 or SEQ ID NO:4; a nucleic acid sequence that encodes a consensus CHIKV E2 protein, wherein the nucleic acid-sequence that encodes CHIKV E2 consensus protein comprises SEQ ID NO:2 or SEQ ID NO:5; a nucleic acid sequence that encodes an immunogenic consensus fragment of consensus CHIKV E2 protein having at least 380 amino acids of CHIKV E2 consensus protein, wherein the nucleic acid-sequence that encodes CHIKV E2 consensus protein comprises SEQ ID NO:2 or SEQ ID NO:5; a nucleic acid sequence that encodes a consensus CHIKV capsid protein, wherein the nucleic acid-sequence that encodes CHIKV capsid consensus protein comprises SEQ ID NO :3 or SEQ ID NO:6; a nucleic acid sequence that encodes an immunogenic consensus fragment of consensus CHIKV capsid protein having at least 225 amino acids of CHIKV capsid consensus protein, wherein the nucleic acid-sequence that encodes CHIKV capsid consensus protein comprises SEQ ID NO:3 or SEQ ID NO:6; and homologues thereof having sequence homology of 95% or more.