发明名称 |
METHOD FOR PREPARATION A THERMOSENSITIVE COATING SUBSTRATE, THE SUBSTRATE WITH A THERMOSENSITIVE COATING AND ITS APPLICATION |
摘要 |
The invention presented in this application relates to a substrate with a thermosensitive polymer layer of changeable affinity for water (LCST) within 0-80°C and the method by which it is made through the immobilization of a thermosensitive polymer from a group of homo- and copolymers of 2-substituted-2-oxazoline onto a modified surface consisting of a non-organic base substrate, preferably glass or silicon. The thermosensitive polymer is immobilized onto the substrate surface by the grafting of 2-substituted-2-oxazoline polymer chains and making the layer covalently bonded with the base substrate through the functional group of the silyl linker as indicated in formula 2 or 3. The polymer immobilization onto the base substrate is performed through grafting that initiates a cationic polymerization from the substrate or through termination of the polymerization by the functional groups on the substrate until a polymer brush conformation with a polymer grafting density of 0.1-1.8 chain/nm 2 is formed. The substrate with the thermosensitive layer made of a polymer from a group of homo- and copolymers of 2-substituted-2-oxazoline is used for cell culture, particularly skin cell culture. |
申请公布号 |
PT2574664(E) |
申请公布日期 |
2014.10.03 |
申请号 |
PT20110460052T |
申请日期 |
2011.09.30 |
申请人 |
CENTRUM MATERIALÓW POLIMEROWYCH I WEGLOWYCH PAN;CENTRUM LECZENIA OPARZEN;SLASKI UNIWERSYTET MEDYCZNY |
发明人 |
ALICJA UTRATA-WESOLEK;WOJCIECH WALACH;NATALIA OLESZKO;ANDRZEJ DWORAK;BARBARA TRZEBICKA;AGNIESZKA KOWALCZUK;JACEK ANIOL;MARTA LESIAK;ANNA SITKOWSKA;ALEKSANDER L. SIERON;MAREK KAWECKI;JUSTYNA GLIK;AGNIESZKA KLAMA-BARYLA;MARIUSZ NOWAK |
分类号 |
C12N5/00;C08G73/02;C09D179/02 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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