| 发明名称 |
MUTANT PROTEASE BIOSENSORS WITH ENHANCED DETECTION CHARACTERISTICS |
| 摘要 |
A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule. |
| 申请公布号 |
US2014298500(A1) |
申请公布日期 |
2014.10.02 |
| 申请号 |
US201414269669 |
申请日期 |
2014.05.05 |
| 申请人 |
Promega Corporation |
发明人 |
Binkowski Brock;Butler Braeden;Encell Lance P.;Fan Frank;Hook Brad;Otto Paul;Vidugiris Gediminas;Wigdal Susan;Zimmerman Kristopher |
| 分类号 |
C12N9/02;A01K67/027 |
主分类号 |
C12N9/02 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a peptide linker linking the C-terminal portion of the circularly-permuted thermostable luciferase to the N-terminal portion of the circularly-permuted thermostable luciferase, wherein the modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase, the peptide linker comprising a sensor region capable of interacting with a target molecule in a cell, wherein the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to at least one of:
i) the parental circularly-permuted thermostable luciferase in the presence of the target molecule, or ii) the modified circularly-permuted thermostable luciferase in the absence of the target molecule, wherein the modified circularly-permuted thermostable luciferase comprises a substitution of an amino acid at a position that corresponds to position 507 of SEQ ID NO:2. |
| 地址 |
Madison WI US |
