发明名称 |
Use Of siRNA To Achieve Down Regulation Of An Endogenous Gene In Combination With The Use of A Sense Construct To Achieve Expression Of A Polynucleotide |
摘要 |
The present invention relates to the combinatorial use of an siRNA targeted against an endogenous gene to knock out or knock down expression of the endogenous gene in a host and a delivery of a polynucleotide encoding the gene in a delivery vehicle/expression vector to the host to provide expression in the host of the protein encoded by the polynucleotide. |
申请公布号 |
US2014296320(A1) |
申请公布日期 |
2014.10.02 |
申请号 |
US201414190884 |
申请日期 |
2014.02.26 |
申请人 |
Dondero Richard S.;Thompson John E.;Taylor Catherine A. |
发明人 |
Dondero Richard S.;Thompson John E.;Taylor Catherine A. |
分类号 |
C12N15/113;C12N15/85 |
主分类号 |
C12N15/113 |
代理机构 |
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代理人 |
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主权项 |
1. A composition comprising a complex of an eIF5A1 siRNA targeted against the 3′ end of eIF5A1, an expression vector comprising a polynucleotide encoding a mutant eIF5A1 wherein the mutant eIF5A1 is unable to be hypusinated, and wherein the siRNA and the expression vector are complexed to polyethylenimine to form a complex. |
地址 |
Riverdale NJ US |