AN EX VIVO HUMAN MULTIPLE MYELOMA CANCER NICHE AND ITS USE AS A MODEL FOR MULTIPLE MYELOMA
摘要
The described invention provides an ex vivo dynamic multiple myeloma (MM) cancer niche contained in a microfluidic device. The dynamic MM cancer niche includes (a) a three- dimensional tissue construct containing a dynamic ex vivo bone marrow (BM) niche, which contains a mineralized bone-like tissue containing viable osteoblasts self-organized into cohesive multiple cell layers and an extracellular matrix secreted by the viable adherent osteoblasts; and a microenvironment dynamically perfused by nutrients and dissolved gas molecules; and (b )human myeloma cells seeded from a biospecimen composition comprising mononuclear cells and the multiple myeloma cells. The human myeloma cells are in contact with osteoblasts of the BM niche, and the viability of the human myeloma cells is maintained by the MM cancer niche.
申请公布号
WO2014159707(A1)
申请公布日期
2014.10.02
申请号
WO2014US24847
申请日期
2014.03.12
申请人
HACKENSACK UNIVERSITY MEDICAL CENTER;THE TRUSTEES OF THE STEVENS INSTITUTE OF TECHNOLOGY
发明人
LEE, WOO;ZHANG, WENTING;TOLIA, PETER;WANG, HONGJUN;ZILBERBERG, JENNY;SIEGEL, DAVID, SAMUEL