METHOD, COMPOSITION AND KIT OF VISUALISATION AND CHARACTERIZATION OF THE NERVOUS SYSTEM BY COMBINING THE STAINING FOR METAL IMPREGNATION AND IMMUNOHISTOCHEMISTRY

摘要

A method of visualization and characterization of the nervous system, which combines two techniques for tissue staining, mercuric impregnation and immunohistochemistry, is described. The method disclosed makes compatible one with the other procedures of staining by carrying them out on tissues subjected to adequate fixing procedures. Thus, the method makes feasible to combine morphometric analysis, which can typically be carried out with mercuric impregnation, with antigenic and/or neurochemical cellular characterization, which can typically be obtained with immunohistochemistry. The method has proven simple, reliable, and suitable for both conventional microscopy and confocal laser scanning microscopy.

主权项

1. A method for visualization and characterization of the nervous system characterized by combining the staining for metal impregnation and for immunocytochemistry comprising at least the steps of:
fixing nervous tissue specimens with an aqueous solution comprising paraformaldehyde in a concentration comprised from 1 to 4% w/v; subjecting said fixed nervous tissue specimens to metal impregnation; treating cut sections of the metal impregnated tissue specimens with suitable reagents for the intended immune-histochemical staining.