发明名称 |
BIOLOGICAL ASSAY OF PEPTIDOGLYCANS |
摘要 |
The present invention relates to a biological method for assaying peptidoglycans (PGN) in a sample, particularly a sample of glucose polymers. The PGN assay includes: a) treating the glucose polymer sample by sonication, heating, and/or alkalizing; b) placing the treated sample or a dilution thereof in contact with a recombinant cell expressing an exogenous TLR2 (toll-like receptor 2) and a reporter gene directly dependent on the signaling pathway associated with the TLR2. The reporter gene codes for a colored or fluorescent protein or for a protein the activity of which is measurable with or without a substrate; c) measuring the reporter gene signal; and d) determining the amount of PGN in the sample using a standard curve of the correlation between the amount of PGN and the strength of the reporter gene signal. |
申请公布号 |
WO2014154651(A1) |
申请公布日期 |
2014.10.02 |
申请号 |
WO2014EP55888 |
申请日期 |
2014.03.25 |
申请人 |
ROQUETTE FRERES |
发明人 |
LANOS, PIERRE;BIGUET, MARC;BERNARD, ROSELYNE;ALLAIN, FABRICE;CARPENTIER, MATHIEU;DENYS, AGNÈS;HACINE-GUERBI, HÉLA |
分类号 |
G01N33/50 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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