| 发明名称 |
Method for preparing nucleic acid library, its uses and kits |
| 摘要 |
Provided are a method of constructing a nucleic acid library, a method of determining a nucleic acid sequence of a nucleic acid sample, and a kit thereof. The method of constructing the nucleic acid library includes the following steps: subjecting a nucleic acid sample to a DOP-PCR amplification, to obtain a first PCR amplification product; subjecting the first PCR amplification product to a second PCR amplification using a DOP-Amp primer, to obtain a second PCR amplification product; and subjecting the second PCR amplification product to an adaptor-ligation PCR, to obtain a third PCR amplification product, wherein the third PCR amplification product constitutes the nucleic acid library. |
| 申请公布号 |
US2014296084(A1) |
申请公布日期 |
2014.10.02 |
| 申请号 |
US201214352492 |
申请日期 |
2012.10.16 |
| 申请人 |
BGI Shenzhen Co., Limited ;BGI Shenzhen |
发明人 |
Yin Xuyang;Zhang Chunlei;Jiang Hui;Zhang Xiuqing;Chen Shengpei |
| 分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of constructing a nucleic acid library, comprising following steps of:
subjecting a nucleic acid sample to a DOP-PCR amplification, to obtain a first PCR amplification product; subjecting the first PCR amplification product to a second PCR amplification using a DOP-Amp primer, to obtain a second PCR amplification product; and subjecting the second PCR amplification product to an adaptor-ligation PCR, to obtain a third PCR amplification product, wherein the third PCR amplification product constitutes the nucleic acid library. |
| 地址 |
Shenzhen CN |
