发明名称 |
ELISA Detection of Urine DEK to Predict and Diagnose Bladder Cancer in Humans |
摘要 |
The present invention is directed to a method of detecting a DEK protein in a human urine sample using an ELISA assay. Methods and compositions for detection of DEK using mAb 260-6F9F6 (as detection antibody) and mAb 16-2C9C3 (as capture antibody) in human urine are provided herein. Specifically, the ELISA assay utilizes a capture mAb and a detection mAb to yield a high sensitivity of <50 ng/mL. The presence of DEK in urine is useful in predicting or diagnosing the occurrence of bladder cancer in humans. |
申请公布号 |
US2014295463(A1) |
申请公布日期 |
2014.10.02 |
申请号 |
US201414258122 |
申请日期 |
2014.04.22 |
申请人 |
Datta Antara;Trama Jason |
发明人 |
Datta Antara;Trama Jason |
分类号 |
G01N33/574;C07K16/18 |
主分类号 |
G01N33/574 |
代理机构 |
|
代理人 |
|
主权项 |
1. An isolated monoclonal antibody (mAb 16-2C9C3), said mAb 16-2C9C3 comprises: (i) CDR1 of the heavy chain variable region which has the amino acid sequence of SEQ ID NO:15; (ii) CDR2 of the heavy chain variable region which has the amino acid sequence of SEQ ID NO:16; (iii) CDR3 of the heavy chain variable region which has the amino acid sequence of SEQ ID NO:17; (iv) CDR1 of the light chain variable region which has an amino acid sequence of SEQ ID NO: 23; (v) CDR2 of the light chain variable region which has the amino acid sequence of SEQ ID NO:24; and (vi) CDR3 of the light chain variable region which has the amino acid sequence of SEQ ID NO:25. |
地址 |
Piscataway NJ US |