METHOD AND APPARATUS FOR SUPER RESOLUTION IMAGING BASED ON FLUORESCENCE RESONANCE ENERGY TRANSFER

摘要

One embodiment of the present invention relates to a method and a system for super resolution imaging based on fluorescence resonance energy transfer. The method for super resolution imaging based on fluorescence resonance energy transfer includes a step of selecting an appropriate FRET (Fluorescence resonance energy transfer) pair and setting a suitable microscope environment; a step of injecting a donor among a pair of FRET donor in a sample to be observed; a step of injecting or expressing an acceptor among FRET pairs around the donor; a step of recording the luminescence signal of the sample injected with the FRET pair; and a step of generating a fluorescent image by analyzing the luminescence signal.