The present invention divides a section of a biological tissue into fragments in a size of including a sufficient amount of cells with a simple structure and a simple operation. The present invention provides a tissue dividing apparatus including: a substrate dividable into a plurality of small pieces (20a), and having a surface to which a section (A) of a biological tissue can be pasted; and a dividing unit (22, 23) that divides the substrate into a plurality of small pieces (20a), and thereby divide the section (A) pasted on the surface into fragments each having a substantially equivalent shape to a shape of each small piece (20a).