DETECTION OF LCAT ACTIVITY

摘要

The present disclosure relates to methods for detecting and measuring the activity of lecithin:cholesterol acyltransferase (LCAT) in solution (e.g. serum, plasma, cell culture media, aqueous solution) with fluorescent sterol substrates. The present disclosure also relates to a method for evaluating efficacy of a therapeutic agent for stimulating LCAT and for determining endogenous LCAT activity in a patient. Also disclosed are kits that are used to carry out the aforementioned methods and methods.