发明名称 |
NUCLEIC ACID ASSEMBLY SYSTEM |
摘要 |
<p>The present invention relates to a method for the preparation of a library of host cells, a plurality of which comprise an assembled polynucleotide at a target locus, which method comprises: (a) providing a plurality of polynucleotides comprising two or more polynucleotide subgroups, wherein: (i) a plurality of polynucleotides in each polynucleotide subgroup comprises sequence encoding a peptide or polypeptide and/or a regulatory sequence; (ii) a plurality of peptides or polypeptides encoded by, or a plurality of regulatory sequences comprised within, each polynucleotide subgroup share an activity and/or function; (iii) at least one polynucleotide subgroup comprises at least two non-identical polynucleotide species; (iv) a plurality of polynucleotides of each polynucleotide subgroup comprises sequence enabling homologous recombination with a plurality of polynucleotides from one or more other polynucleotide subgroups; and (v) a plurality of polynucleotides in two polynucleotide subgroups comprise a nucleotide sequence enabling homologous recombination with a target locus in host cells; and (b) assembling the plurality of polynucleotides at the target locus by homologous recombination in vivo in host cells, thereby to generate a library of host cells, a plurality of which comprise an assembled polynucleotide at the target locus. The assembled polynucleotides may be recovered, thereby to prepare a library of nucleic acids.</p> |
申请公布号 |
EP2783000(A1) |
申请公布日期 |
2014.10.01 |
申请号 |
EP20120788597 |
申请日期 |
2012.11.23 |
申请人 |
DSM IP ASSETS B.V. |
发明人 |
ROUBOS, JOHANNES, ANDRIES;MEIJRINK, BERNARD;KERKMAN, RICHARD;DULK, DEN, BEN |
分类号 |
C12N15/10;C12N15/52;C40B40/06;C40B40/08 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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