| 摘要 |
The invention relates to chemistry and biotechnology, in particular to the synthesis of a new coordinative compound of cobalt(III) and a process for cultivation of Porphyridium cruentum microalga with the use thereof.According to the invention, a coordinative compound - bis(dimethylglyoximato)chloro(isonicotinoylhydrazone-2-hydroxy-1-naphthaldehyde)cobalt(III) is claimed.It is also claimed a process for cultivation of Porphyridium cruentum microalga, consisting in that microalga is cultivated on a nutrient medium, comprising in g/L: NaNO3 - 5,0; NaCl - 7,0; KCl - 7,5; MgSO4·7H2O - 1,8; Ca(NO3)2·4H2O - 0,15; KBr - 0,05; KI - 0,05; K2HPO4 - 0,2; FeCl3·6H2O - 0,0027; ZnSO4·5H2O - 0,00002; CuSO4·5H2O - 0,00005; MnSO4·5H2O - 0,0003; H3BO3 - 0,0006; MoO3 - 0,00002; NaVO3 - 0,00005; bis(dimethylglyoximato)chloro(isonicotinoylhydrazone-2-hydroxy-1-naphthaldehyde)cobalt(III) compound - 0,010…0,014 g/L and distilled water up to 1 L, having a pH of 6.8…7.2; at the temperature of 23…25°C and the lighting of 2000…3000 lx/cm2.The result is to increase the amount of lipids and eicosapentaenoic acid in the absolutely dry biomass of microalga. |
