| 摘要 |
The invention relates to a method for quantitative determination of test results from diagnosis methods with the aid of an optoelectronic evaluation appliance, and to the evaluation appliance itself, characterized in that the digital pixel information per color level or grey level is represented in its intensity in the microprocessor as one column per pixel, wherein the column height corresponds to the intensity, and these columns are displayed alongside one another on one plane, such that the intensity distribution is displayed over the test area as a surface contour or surface profile, the height profile of which corresponds to the intensity profile of the color intensity received by the CCD. Fields of application for the invention are test methods in biochemical laboratories, such as medical diagnosis, forensic medicine, foodstuff diagnosis, molecular biology, biochemistry, gene technology and all other related fields, as well as patient monitoring for home users or in pharmacies. |
| 主权项 |
1. Method for evaluation of a test strip with an appliance containing an insert, an optic portraying the image of the test strip onto a receiver, reference fields or reference images, as the case may be, a reception component, lighting for the test strip, evaluation electronics containing an evaluation algorithm, a display, an energy supply and electrical interfaces as well as an output device, wherein for the evaluation of a test strip on which body-specific substances, in particular proteins released in a certain incident in the body, are detected with the help of labelled antibodies, the concentration of the labelled antibodies is determined by a colour change in a strip, wherein the colour intensity (grey level) of the concentration of the antibodies per volume is proportional, the test strip is portrayed with the help of an optic on a CCD comprising areally arranged pixels on one level (individual photo diodes), wherein the intensity in certain directly portrayable colour or grey levels is used for the evaluation and the relative discoloration is compared via calibration values deposited in the programme, wherein the digital pixel information per colour or grey level in the microprocessor is portrayed in its intensity as a column per pixel, wherein the height of the column matches the intensity, an evaluation algorithm is carried out, firstly finding the test strip by scans through the peaks running orthogonal to one another (maximum), defining an area defining the test strip as areas to be detected and integrating over the colour value levels of all these pixels and forming the mean value and these columns being portrayed next to one another on one level such that the distribution of the intensity over the test field is portrayed as a surface contour or surface profile, the sequence of height of which matches the course of intensity of the colour intensity received with the CCD, and reference fields and reference images located next to the test field to be detected on the optical object level of the appliance are recorded by the CCD simultaneously with the image of the sight field of the test with capture line and control line, wherein possible error and aging processes are eliminated by the reference fields and reference images in the appliance by a definition of an area for the white balance being carried out, via which there is also integration and thereafter a difference being formed between pixel integration value and white balance and the value being compared with calibration values deposited in a database and being output as a measured value. |
