Pneumatic alternating pressure membrane cell separation system

摘要

Provided are filtration systems that can be referred to as Pneumatic Alternating Cell Separator (PACS), useful components thereof in the form of assemblies or kits of parts that can be used to build the system, and use of the system for filtering fluids, for instance, in cell culture perfusion systems comprising a filter-containing chamber, an expansion chamber and a gas flow controller.

主权项

1. A fluid filtration system comprising:
a) a liquid containing bioreactor; b) a filter-containing compartment; c) a liquid transfer line connecting the storage vessel to an entrance end of a filter-containing compartment, which liquid transfer line is capable of directing the liquid from the bioreactor into the entrance end of the filer-containing compartment; d) a expansion chamber with at least two openings, wherein a first opening is connected at an exit end of the filter-containing compartment and wherein a second opening is connected to a gas flow controller, wherein the expansion chamber contains no separation means between the first and second openings, and wherein at least one level sensor is mounted on or in the expansion chamber to indicate the level of fluid in the expansion chamber; e) a gas flow controller in communication with the level sensor for alternatively providing the expansion chamber with positive qas pressure and negative pressure; and f) a liquid harvest port connected to the filter-containing compartment for removing filtered liquid from the filter-containing compartment.