Plant transformation without selection

摘要

The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.

主权项

1. A method for identifying transgenic corn plants, comprising:
(a) obtaining corn plant cells transformed with a DNA segment comprising a nucleic acid sequence of interest; (b) regenerating a plurality of corn plants or differentiated corn plant parts from the cells without first selecting or screening for the presence of a functional genetic component which produces a product that serves to identify a transformed plant, or for the presence of said DNA segment; and (c) identifying at least a first transgenic corn plant or transgenic differentiated plant part from the plurality of corn plants or differentiated corn plant parts, wherein the plants or differentiated corn plant parts are regenerated by growth solely on liquid media prior to identifying the transgenic corn plant or transgenic differentiated plant part and wherein the corn plants or corn plant parts are regenerated not later than 6 weeks after the DNA segment is transformed into the corn plant cells.