Method for identifying immune response modulators

摘要

Methods of identifying immune response modulators are disclosed. Some methods comprise identifying chemical candidates that modulate oligomerization of FOXP3 and/or fragments thereof comprising the Zinc-LeuZip domains. Some methods comprise identifying chemical candidates that modulate the hetero-oligomerization of FOXP1 with FOXP3 and/or fragments thereof comprising the Zinc-LeuZip domains. Some methods comprise identifying chemical candidates that modulate interaction of IL-2 promoter with FOXP3 and/or fragments thereof comprising the Zinc-LeuZip domains. Method of treating individuals who have or are suspected of having autoimmune disease, inflammatory disease, cell, tissue or organ transplantation, or coronary artery disease, and methods of treating individuals who have or are suspected of having infectious disease, cancer, or who are immunocompromised or undergoing vaccination are disclosed.

主权项

1. A method of identifying an immune response modulator, the method comprising:
a) isolating
i. a FOXP3 protein, or a fragment thereof having a Zinc-LeuZip domain, or a polynucleotide encoding said FOXP3 protein, or a fragment thereof having a Zinc-LeuZip domain;ii. HDAC7 protein or TIP60 protein, or a polynucleotide encoding said HDAC7 protein or TIP60 protein; andiii. a nucleic acid comprising an IL-2 promoter operably linked to a coding region; b) combining the isolated substances from step a; c) measuring transcription of the IL-2-linked coding region; and d) comparing the level of transcription of the IL-2-linked coding region that occurs in the presence of a chemical candidate to the level of transcription of the coding region that occurs in the absence of the chemical candidate, whereby the difference in level of transcription indicates that the chemical candidate is an immune response modulator.