Carotenoid production in a recombinant oleaginous yeast

摘要

Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing carotenoids (e.g., β-carotene, lycopene, lutein, zeaxanthin, canthaxanthin, astaxanthin) are provided. The strains may also be engineered to co-produce at least one ω-3/ω-6 polyunsaturated fatty acid and/or at least one additional antioxidant. Methods of using the carotenoid products obtained (e.g., biomass and/or pigmented oils) in food and feed applications are also provided.

主权项

1. A pigmented oleaginous yeast biomass comprising a recombinant Yarrowia lipolytica capable of accumulating at least 25 weight percent of its dry cell weight as oil and capable of producing (i) a commercially significant amount of at least one carotenoid compound selected from the group consisting of: astaxanthin, β-carotene, lycopene, zeaxanthin, lutein and canthaxanthin and (ii) high level production of at least one polyunsaturated fatty acid selected from the group consisting of an omega-3 polyunsaturated fatty acid and an omega-6 polyunsaturated fatty acid.