Concentration independent modulation of local micromechanics in a fibrin gel

摘要

An apparatus and method for providing a gradient strain to an extracellular matrix to induce mechanotransduction. The gradient strain can be produced by adhering the extracellular matrix to a post and the walls of a sample holder, and then rotating the post and the sample holder. This strain can result in increased stiffness of the extracellular matrix with very little loss of pore size.

主权项

1. An apparatus for applying strain on an extracellular matrix (ECM) comprising:
a base configured to securely hold a sample container comprising an ECM; a post configured to contact the ECM; and a means for imparting a rotational strain on the ECM comprising a threaded screw, a spring plunger assembly and a lever arm, configured to rotate the post relative to the sample container.