Method for filtering nucleic acids, in particular from fixed tissue

摘要

The invention relates to a method for filtering nucleic acids, to a kit for carrying out the method according to the invention and to a novel use of magnetic particles for filtering a biological sample. The method according to the invention comprises the following steps: a) the sample is held in an aqueous solution; b) lysing of the sample; c) separation of cellular debris; and d) the nucleic acids are isolated from the solution, steps (a) to (c) taking place under non-chaotropic conditions.

主权项

1. A method for purifying nucleic acids from a biological sample, having the following steps:
a) taking up the sample in an aqueous solution; b) lysing the sample; c) separating cell debris comprising proteins and membrane components from the sample by suspending magnetic particles in the solution binding the cell debris to the suspended magnetic particles and using a magnetic field for retaining and separating the magnetic particles and the bound cell debris from the solution; and d) isolating the nucleic acids from the solution; characterized in that steps (a) to (c) take place under non-chaotropic conditions; further characterized in that the solution is heated to at least 50° C. before step (d).