Polypeptides having cellulolytic enhancing activity and nucleic acids encoding same

摘要

The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

主权项

1. An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide having cellulolytic enhancing activity selected from the group consisting of:
(a) a polynucleotide encoding a polypeptide having cellulolytic enhancing activity comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 4; (b) a polynucleotide encoding a polypeptide having cellulolytic enhancing activity comprising a nucleotide sequence that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 3, (ii) the cDNA sequence thereof, or (iii) a full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; and (c) a polynucleotide encoding a polypeptide having cellulolytic enhancing activity comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 3 or the cDNA sequence thereof; wherein the polynucleotide is operably linked to one or more control sequences that are foreign to the nucleotide sequence encoding the polypeptide having cellulolytic enhancing activity.