Haptens, hapten conjugates, compositions thereof and method for their preparation and use

摘要

A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.

主权项

1. A method for detecting a target in a sample, comprising:
contacting the sample with a specific binding moiety that binds specifically to the target, wherein the specific binding moiety is conjugated through an alkylene oxide linker to a pyrazole hapten having the formula wherein R1 is hydrogen, one of R3 or R4 is hydrogen and the other one is carbonyl or amido group and wherein the specific binding moiety is conjugated to the carbonyl or the amido group via said alkylene oxide linker; contacting the sample with an anti-hapten antibody specific for the pyrazole haptein; and detecting the anti-hapten antibody.